روابط خويشاوندي جنس يونجه در ايران بر اساس نشانگر مولكولي RAPD

Phylogenetic relationships in Iranian medicago genus using RAPD marker

Genetic diversity of 54 populations from 22 species of genus Medicago collected from Iranian natural habitat was studied. DNA was extracted from bulked leaf samples of each population and used for RAPD-PCR. RAPD marker produced by 11 UBC random primers was analyzed according to Maximum Parsimony method. Using the computer software PAUP (Swofford 2000) a phylogenetic tree with 5 main cluster was generated. Populations of M. aculeala Mconstricta, M.rigiduloidos and M. rigidula with hard pod walls and spongy texture were classified in one cluster. Populations of M. sauvagei, M. laciniata and M. polymorpha which had soft and flexible pod walls were classified together in a separate cluster. Populations of each species were also classified together in subclusters indicating that RAPD marker were capable of identifying genetic diversity at the species level. Populations of one species grown under similar climatic conditions were grouped together under the cluster of that species. This indicates that genetic diversity correlates with geographical distribution of wide spread annual medic species in Iran. Results of this study showed that RAPD marker are applicable as a complementary tool in taxonomic identification of genus Medicago at both species and population levels.