اندازه گيري آفلاتوكسين ها در ميگوي پرورشي ايران به روش كروتوماتوگرافي مايع با كارآيي عالي

High performance liquid chromatographic determination of aflatoxins in Iranian shrimp

An HPLC method using immunoaffinity column cleanup and post-column electrochemical derivatization with fluorescence detection was developed for the quantization of aflatoxin residues in shrimp muscle . The shrimp homogenate ( 50 g ) is extracted with methanol : Water ( 80%) , and the extract was defatted with hexane , and the aflatoxins were partitioned into methanol: water. The extract wan diluted by water, and the solution is passed through an immunoaffinity. Aflatoxins subsequently eluted and were detected using an HPLC with Fluorescence detection. Mean recoveries of flatoxins (B1 , B2 , G1 , G2) from tissue directly fortified at 1.25 ,2.5 ,5 ,10 and 20 ppb, were over 87% (ranged from 87.7 to 112 %). The within laboratory relative standard deviations [RSDr] were less than 16.6% ( ranged from 4.3 to 16.6%). Subsequently 47 samples of shrimp were collected from the southern provinces of Iran and were analyzed for the presence of and aflatoxins Bl , B2, Gl, and G2. Aflatoxin B2 contamination only detected in one sample at a level of 1.71 ppb . Such a low contamination level may pose a negligible risk to human health.