مقايسه تاثير سميت عنصر روي بر سلول هاي رده لنفوييدي راجي و مولت -4 با تكنيك فلورسانس

Comparison between the cytotoxic effects of zinc on Raji and Molt -4 cell line

Background and purpose : Zinc has important effects on human health, specially on structural and functional activities of immune system. This study was carried out to examine the in vitro cytotoxic effects of Zinc on Raji and Molt-4cell line. Materials and methods : In this study (by cell culture) the cell line was exposed to different concentration of Zinc followed by incubation (37 c, 5% Cot) at various time points (12 to 72 hrs). Yiability and proliferation of Raji and Molt-4 cells were then evaluated with florecent (Ethidium-Bromide and Acridine-Orange) staining. Data were analysed using sPSS (Dunet and analysis of varians test). Results : The results showed different responces to different amount of Zinc by The Raji & Molt-4 cells. Concentrations up to 1001.tM at different incubation time points had no effects on cell line when compared with the controls. With higher concentrations of Zinc (200-500µM) viability diminished significantly at 12, 24, ... hrs of incubation times when compared with the controls (p<0.05). Conclusion : We conclude that Zn has dose-dependent cytotoxic effect on Raji and Molt-4 cells.