بررسي بيوسيستماتيك (مورفولوژي‌، كاريولوژي و مورفومتري‌) دوزيستان بي دم )ANURA( استان مركزي‌

Biosystematic study (morphology, karyology and morphometry) of anuran amphibia in Markazy Province

Anuran amphibian are known vertebrate. They have been studied systematically in Iran, recently. In order to study biosystematics of anura in the Markazy Province and compare with Khorasan Province, 122 specimens were collected from six stations (Arak, Ghadamgah, Shazand,Farmahin, Delijan, Khomein) at spring and summer (2004- 2005). Samples were transfered alive to the zoology lab, identified and were fixed after karyologic studies. Identification based upon karyology and morphologic characters showed that anuran samples belong to marsh frog, Rana ridibunda and green toad, Bufo viridis with a set of 26 (2n=26) and 22 (2n=22) chromosomes, respectively. The phonetic studies between populations of two species, 14 characters in frog and 17 characters in toad were measured. T-test analysis between sexes were significant in 12 characters for frogs and only one character for toads (P<0.001). One way ANOVA between populations for each species were significant in 13 characters for frogs and 6 characters for toads (P<0.001). Difference between multivariate mean vectors confirmed in T2Hotelling for populations and sexes of marsh frog (P<0.001). Length of foot and femur were discriminated distinct sexes based on discrimination analysis with 89 % correct grouping for frogs and space between pellicle with 92% correct grouping for toads. Discrimination analysis between populations discriminated groups based on two functions with 78%coirect grouping in frogs and 88% correct grouping in toads. Cluster analysis based on centroids grouped populations resulted from geographical, ecological and genetical conditions