مطالعه آلودگي به لپتوسپيرا در افراد در معرض تماس مستقيم و غيرمستقيم با حيوانات در سطح شهر اهواز

A study of leptospiral infection among the people of Ahwaz with direct and indirect contact with animal

In order to detect leptospiral antibody in the serum of people in Ahvaz, blood samples were taken from 180 men, and they were divided into direct contact (93 person) and non-direct contact (87 person) with animals. The sera were initially screened at serum dilution of 1:100 against six live antigens of Leptospira interrogans: pomona, canicola, hcirdjo, ballum, icterohemorrhagiae and grippotyphosa using the microscopic agglutination test (MAT). Antibodies against one or more serovars at dilution of 1:100 were detected in 21(22.58%) and 11(12.64%) sera of the men with direct and non-direct contact to animals, respectively. Among the positive sera, antibodies were most frequent in serovar canicola (56%) followed by grippotyphosa (26%), pomona and hardjo (each of them 8%) in the men with direct contact to animals and in the men with non-direct contact to animal, antibodies were most frequent in serovar canicola and grippotyphosa (each of them 41.2%) followed by pomona (11.8%) and hardjo (5.9%). The majority of titre level was 1:100 for all serovars and the frequency of 1:100 were 84% and 70.6%, 1:200 were 16% and 11.8% and 1:400 were zero and 17.7% in the men with direct contact and non-direct contact to animal, respectively. The prevalence of leptospiral antibody in the men with direct contact to animal was higher than the non-direct contact, but statistical analysis showed non significant difference between these groups.