جداسازي‌، تخليص‌، شناسايي و سازش باكتريهاي مزوفيل معدن مس سرچشمه‌

Isolation, Purification, Identification and Adaptation of the Mesophilic Bacteria in Sarcheshmeh Copper Mine

In order to isolate and identify endemic bacteria which affect in bioleaching process on low grade copper sulphide, twenty four samples of water, soil and sludge were collected from various sites of Sarcheshmeh copper mine. These samples were cultured. Bacteria growth was observed on seven samples. Isolation and purification stages were implemented on these samples. Then bacteria were identified using morphological and diffrentional experiments. The total isolated bacteria of seven samples were gram negative Bacilli which were able to oxide the pyrite, elementary sulphur, tiosulphat, copper sulphide and ferrous iron. These oxidations were mainly accompanied by changes on pH and culture medium color. The purified bacteria had the same characteristics of Thiobacillus ferrooxidans bacterium from the point of view of morphological characteristics and differentional experiments. The adaptation of this bacterium to the high concentration of copper was performed on consequently cultures. The adaptated bacterium was a mutated species of endemic T. ferrooxidans which was completely grown on propitiate medium for 24 - 48 hours and was able to tolerate the solute copper up to 15269 ppm.