بررسي ميزان و حذف گوسيپول آزاد در كنجاله هاي تخم پنبه ناحيه خراسان به منظور ارايه يك جيره غذايي مناسب براي طيور گوشتي

Determination and detoxification methods of cottonseed meal gossypol for broiler chicken rations

The following steps were used to evaluate the amount of free gossypol in cottonseed meal (CSM) samples of Khorasan province. Samples of (1-2 kilograms of) CSM were taken from three oil extraction factories in Nyshabor and one in Mashhad. Mashhad cottonseed meal (MCSM) had more crude protcion (%29.3) and less crude fiber (%22.S) than others. therefore this meal was used to study the broiler chicken performance_ Total and free. gossypol content of CSM samples were determined by 11PI.C. For determining the effect of dihydrated Fero-sulfate (DFS) in lowering free gossypol the following treatments were used in the lab: 1 - Control MCSM. (with zero DFS) 2 - MCSM with added DFS (as equal amount of free gossypol) 3 - MCSM with added DES (as equal amount of total gossypo). The broiler performance were studied by a factorially arranged (3 x 4) completely randomized design experiment consisting of four levels of MCSM (0,5,10 and 20% of diet) and three levels of DFS (zero, equal to free gossypol and equal to total gossypol). All the nutrients density based on metabolizable energy were the same in all treatments. Each treatment was consisted of 6 replicates of 8 day-old broiler chickens. Free and total gossypol in Mashhad cotton seed sample were determined to be 0.023% and 0.077% respectively. Addition of DFS equal to free gossypol, lowered free gossypol to %0.0001 and addition of DFS to total gossypol lowered free gossypol to zero. Different levels of DFS and MCSM did not have any significant effect on daily feed intake, live weight, weight gain, feed conversion and mortality of broiler chickens up to 56 days of age (P>0.05).