تكثير درخت سفيد پلت از طريق كشت تخمدان بالغ POPULUSCASPICA

Propogation of Populus caspica tree through mature ovary culture.

In order to mass propagation of Populus caspica, in vitro matured ovary culture was used. For embryo germination, 14 days old capsules, derived from natural and artificial (twig and pot pollination system) pollination were isolated. Isolated capsules were then transferred to MS, Half-MS (Half-concentrated MS) and SS (solidified 3% sucrose solution) hormone free medium for embryo germination. Significant differences (p<0.01) were observed between media and two kind of pollination for mean number of plantlets indication per isolated capsule. High number of plantlets was observed per capsule in half- MS medium. The artificial pollination of catkin showed higher number of plantlets production per capsule than that of the occurring natural pollination (24.77 and 19.46 respectively). Plantlets with 5 or 8 cm height were then cultured in the same medium within jars, before being transferred to the potting soil. More than one thousand plantlets were produced from both kind of pollination. Successfully acclimatized plantlets in green-house were then transplanted to the field.