چكيده لاتين :
Introduction: Helicobacter pylori colonize in gastric mucosa and causes some disorders in upper gastrointestine. The clinical outcome of Helicobacter pylori infection may be associated with or without cagA.
Materials & Methods: To investigate presence of cagA gene in Helicobacter pylori strains among patients with NUD, Peptic Ulcer and Gastric Cancer, 150 gastric biopsy specimens were taken. Biopsy samples were cultured on selective media under microaerophilic condition. After incubation period, the colonies were diagnosed by biochemical tests. Finally, using unique primers, cagA gene was investigated among the Hpylori strains.
Results: Among the samples, Hpylori was isolated in 92 cases by culture method. After PCR method was done on culture positive samples,it was found that the prevalence of cagA gene in strains isolated from patients with NUD, Duodenal ulcer, Gastric ulcer and Gastric Cancer was 64.7% (22 from 34 cases ), 100% (28 from 28 cases), 90% (18 from 20 cases) and 100% (10 from 10 cases) respectively. In this study, there was a significant different between association of cagA gene in strains isolated from patients with peptic ulcer and gastric cancer (P<0.05), but this difference was not significant among strains isolated from NUD patients (P>0.05).
Conclusion: According to the findings, it is suggested that presence of cagA gene in
strains of Helicobacter pylori may play an important role in aggravating disorders in Iran.
