مقايسه پروتئين هاي سيتوپلاسمي جدايه هاي فوزاريوم سولاني بدست آمده از هوا و منابع غذايي مناطق مختلف ايران به روش SDS-PAGE

Comparison of the cytoplasmic proteins of Fusarium so/ani isolate, obtained from air and food sources in Iran by SDS - PAGE

Studies have shown that various species of Fusarium grow well in food resources. specially in agricultural crops like cotton meal, rough rice, potato and wheat. Also by distributing conidia in air and inhaling them, they can act as pathogen, saprophyte or allergenic agents. Fusarium so/ani is known as soil saprophytic and it has allergenic components in cell wall and cytoplasm of conidia and hyphae, used for diagnostic and allergy therapy purposes. The goal of this study was to identify the cytoplasmic proteins of Fusarium so/ani isolates under study and evaluate the correlation and differention of the antigens between isolates. In the present study, 12 samples of Fusarium solani, with sources of air and foods, were maintained at collection of mycology center of faculty of Veterinary Medicine of Tehran University. They were broken by Freez and Taw and mechanical methods, and after centrifuging. supernatants which consist of most cytoplasmic proteins were assessed by SDS-PAGE technique. The results showed that 12 Fusarium solani isolates have common and uncommon protein bands and some isolates have special protein bands such as 87, 40 and 108 KD, in which specifics for isolates were 3, 4 and 10 respectively. Also, the bands of 24 and 32 KD were presented on the all isolates. In cornparision with two standard strains, UAMH 7419 and UAMH 3317, the 24 KD band is specific for Iranian isolates, but 32 KD band was presented in standard strains. Therefore, the 24 KD band is special protein in Iranʹ s native samples. This study suggests that for diagnostic and vaccine preparation and allergy therapy, the specifie native Fusarium so/ani antigens should be used by researchers.