بررسي تاثير ساليسيليك اسيد برتغييرات پروتيين القا شده در كشت بافت گياه ميخك Dianthus Caryophyllus L

Salicylic acid induced proteins in Dianihus caryophyllus L. shoot culture

Background: The purpose of this study was to achieve defense proteins. Indeed, it proposed that salicylic acid (SA) involved a number of defense mechanisms. The influence of SA application probably on changes in levels of protein extent of Dianthus shoots was investigated. Methods: Firstly, explants of cv. Cerise Royallette were cultured on OM medium with 0.2 mg.L 1 NAA, 0.5 mg.L"1 BAP, supplemented by 0, 10, 50, and 200 fiM SA. After 4 weeks, growth of shoots were measured and then prepared extracts and performed Bradford test. These substances were tested with SDS-PAGE, to find the concentrations which caused changes in quality of proteins. Results: Data analysis indicated that the most typical effect of SA treatment protein content of shoots was occurred. Indeed, treatment increased protein content considerably, however, it decreased the number of shoots. Analysis of the polypeptide pattern following SDS-PAGE showed that all tested SA changed the accumulation of both 38 and 39 kDa polypeptides that located very close to each others, and the level of their accumulation was found to be varied depending on the kind and intensity of the stressor. Conclusion: The data provide evidences that plant defense mechanisms might involve the same proteins in response to SA and may lead to induction or deletion of defense proteins changes.