مقايسه رقيق كننده هاي مختلف اسپرم خروس بومي در رقت هاي مختلف اسپرم طي دو زمان تلقيح

A Comparison of Various Extenders of Isfahan s Rooster Sperm of DifferentDilution Over Two Dilution Periods

The present study was conducted in order to determine the proper extender for poultry semen among Beltsville, Ringer and milk and the comparison of various proportions of different extenders applied for dilution (semen-over-extender ratios 1:1 and 1:2) and its impact on fertility and semen storage period (non-storage or 24-hour storage). This study was carried out in the Isfahan Center for Breeding and Production of Native Hens. The study consisted of eleven treatments and each treatment included three roosters as replicate and 24 hens. Collected semen was diluted in 1:1 and 1:2 ratios by various extenders and insemination was conducted immediately or after a 24-hour storage period at 5 degrees of centigrade. The results indicated that following a 24-hour storage period, fertility declined and that the effect of various insemination periods on fertility proved to be significant (P<0.01). There was no significant difference between the 1:1 and 1:2 ratios for the extenders (P<0.05). Among the extenders, when using non-stored semen, employment of the 1:1 Ringer extenders produced the highest fertility and there was no significant difference between the Beltsville and control extender. However, using milk as extender lowered the fertility. In conclusion, none of the applied extenders are proper for semen storage and application of Ringer as extender resulted in the highest fertility.