تنوع ژنتيكي برخي توده هاي سير Allium sativum L. ايران با استفاده از نشانگرهاي RAPD

Genetic Diversity Among Some Iranian Garlic Genotypes (Allium sativum L.) Using RAPD Markers

RAPD markers were employed to determine genetic diversity among 39 garlic populations. In total 45 primers were used for PCR reactions on the template DNAs, among which 26 showed good amplification and polymorphism. These primers produced 185 bands, among which, 113 were polymorphic. Cluster analysis of the genotypes was performed based on either the presence (1) or absence (0) of the bands, using Jaccardʹs similarity coefficient and Complete method. The highest similarity (84%) was detected between two genotypes both of which were from Tarom of Zanjan province and two genotypes from Dezfool and Ahvaz (both from Khuzestan province). Lowest similarity (0.26%) was observed between two genotypes from Behbahan and Rasht, and one from Arak at a distance of 0.50 similarities on the dendrogram. The genotypes were divided into 10 sub-clusters, mostly in accordance with their specific morphological traits while some on their geographical origin. Cophenetic coefficient of r=0.9 between similarity matrices and the dendrogram showed the goodness of fit for the dendrogram as well a for similarity data. According to the obtained results, the studied populations were of a high genetic diversity, showing the richness of garlic resources in Iran.