بررسي تاثير سطوح مختلف ويتامين هاي E و C در رقيق كننده هاي شير و تريس بر خصوصيات اسپرم قوچ آتاباي در شرايط منجمد

Investigation the effect of various levels of Vitamins E and C in milk and tris extenders on characteristics of Atabay ram semen in frozen condition

The objective of present study was to investigate the effect of various levels of Vitamins E and C in milk and tris extenders on Atabay ram semen characteristics in frozen condition. Semen were collected from 6 Atabay rams using artificial vagina and suitable samples were mixed together and subjected to different treatments in milk and tris extenders. Spermatozoa characteristics including percentage of motile and viable cells were assessed by electronic microscope. This experiment was carried out in 3x3 factorial arrangement on theʹ basis of completely randomized design. First factor was various levels of Vitamin E (0, 30 and 60 ug/rnl) and second factor was various levels of Vitamin C (0, 150 and 300 ug/ml). Results indicated that in frozen condition, the effect of Vit. E on motility and viability percentage of spermatozoa in milk and tris extenders was significant (P0.05). Mean comparison on the basis of Duncan test showed that the highest motility of spermatozoa in tris (48.48±O.087) and milk extenders (46.45±O.085) were obtained in level 30 ug/ml ViI. E. In conclusion, use of Vitamin E (30 ug/ml) in milk and tris extenders is recommended for storage of Atabay ram semen in frozen condition.