شناسايي مواد تشكيل دهنده و بررسي اثرات آنتي اكسيداني اسانس و عصاره متانولي گياه كاكوتي كوهي (Ziziphora clinopodioides Lam) در مرحله قبل از گلدهي

Composition and Antioxidant Activity of the Essential Oil and Methanolic Extract of Ziziphora Clinopodioides Lam in Preflowering Stage

Background & Aims: Ziziphora clinopodioides Lam belongs to the Labiatae family and its dried aerial partsare usedas flavors and also in the treatment ofcoldand cough. Methods: The chemical composition of the essential oil obtained from aerial parts by hydro distillation method was analyzed by GC and GCIMS. The methanolic extractwas also obtained from the aerial parts of the plant. Samples were subjected to a screening for their possible antioxidant activities by using 2, 2diphenyl- I-picrylhydrazyl (DPPH) and p-carotene-linoleic acid. Results: Twenty three constituents accounting to 96.6% ofthe total oil were identified. Major components of the oil were pulegon (30.1%), thymol (21.3%), p-mentha-3-en-8-o1 (12.9%). piperitenone (9.3%)and I, 8-cineol (4.1%), respectively. In the DPPH test system, the ICso value for antioxidantactivity ofmethanolic extractwas determined to be 28.4J.lglml, whereas itwas 55.3 ug/ml for the oil. In p-carotene-linoleic acid test system, antioxidant activities ofthe extractand oil were 89.3%and 61.6%,respectively. Conclusion: In general, methanolic extract of ziziphora clinopodioides lam showed greater antioxidant activity than its essential oil.