پيوند سلول هاي بنيادي اسپرماتوگوني در موش

Spermatogonial Stem Cell Transplantation in Mice

Background: Spermatogonia are the only male gerrnline stem cells that can proliferate in adulthood and offer intere sting applications, as they are potentially totipotent and immortal cells . Tran splantation of germ cells leads to restoration of spermatogenesis from spermatogonial stem cell s. Testis cell transplantation from a mouse into a recipient mouse ʹ s seminiferous tubules result s in donor-cell-derived spermatogenesis in nearly all the hostsʹ testes. Methods: To isolate germ cells, a two to 4-day old mouse testis cell s were dissociated enzymatically. To evaluate transplantation success , the recipi entʹs testis should have no or negli gible endogenous spermatogenesis. Therefore, busulfan treated mice were used as recipients. To distinguish the progeny of the tran splanted donor stem cells from endogenous germ cells, BrdU-labeled cells were used. Results: Transplantation of stem cells into seminiferous tubules of the recipientʹs left testis was done and cryosection of the transplanted testis showed a significant increase in the number of spermatozoa in the left epididyma l lumen compared with that of the control (The right testis ). C-kit gene was expressed in spermatogonial stem cells but alkaline phosphatase was not. Conclusion: Culture of spermatogonial stem cells before transplantation help s proliferation and impr oves stem cell tran splantation efficiently. The present study confirms that regeneration after cytotoxic treatment is based on stem cell expansion, as ori ginally proposed , based on morphological criteri a. The results of this study provided functional data in support of stern cell self-renewal, and demonstrat ed the increase in the number of stem cell during regeneration upon transplantation.