بررسي بيان ژن تيروگلوبولين در آندومتر موش هاي ماده Balb/c در مراحل مختلف سيكل استروس

Evaluating thyroglobulin gene expression in the endometrium of Balb/c female mice during estrous cycles

Background: In pregnant women with anti-thyroglobulin antibodies, the prevalence of abortion is 2-4 folds higher compared to normal controls. The direct effects of such harmful autoantibodies on female reproductive organs may serve a role in pregnancy loss. To address this hypothesis, expression of thyroglobulin gene in the endometrium of cycling mice has been evaluated in this study. Methods: Stages of estrous cycles were determined by examining vaginal cytology of Balblc mice. At each phase, the mice were killed and their and endometria were carefully pealed away from underlying tissues. Expression of thyroglobulin- specific transcript was investigated by two sets of primers using reverse rranscriptase-polymerase chain reaction CRT-pe R), In addition, polyclonal anti-thyroglobulin antibodies were produced aud expression of thyroglobulin in the reproductive tissues of female mice was evaluat ed through immunohistochemistry. Results: The results showed that thyroglobulin mRNA was expressed neither in the endometria nor in the estrous stages of the cycling mice. Immunohi stochemistry analysis confirmed the results in that thyroglobulin or its cross reactive-antigens were not expressed at the protein level in the female reproductive organs including the uterus, fallopian tubes or ovaries. Conclusion : The results showed, for the first time, that thyroglobulin was not expressed in the reproductive orga ns of female mice. It is plausible that anti-thyroglobulin antibodies could interact with newly-generated antigens during placentation and pregnanc y.