بررسي انحراف هاي كروموزومي در كارسينوم داكتال مهاجم پستان به روش هيبريديزاسيون ژنومي مقايسه اي

Chromosomal aberrations detected by comparative genomic hybridization technique (CGH) in invasive ductal carcinoma of breast

Background: Nonlethal genetic damage is the basis for carcinogenesis. As various gene aberrations accumulate, malignant tumors are formed, regardless of whether the genetic damage is subtle or large enough to be distinguished in a karyotype. The study of chromosomal changes in tumor cells is important in the identification of oncogenes and tumor suppressor genes by molecular cloning of genes in the vicini ty of chromosomal aberrations, Furthermore, some specific aberrations can be of great diagnostic and prognostic value. Comparative genomic hybridizati on (CGH) is used to screen the entire genome for the detection and/or location chromosomal copy number changes. Methods: In this study, frozen sections of 20 primary breast tumors diagnosed as invasive ductal carcinoma from the Cancer Institute of Imam Khomeini Hospital, Tehran, Iran, were studied by CGH to detect chromosomal aberratio ns. We compared histopathological and immunohistochemical findings. Results: Hybridization in four of the cases was not optimal for CGH analysis and they were excluded from the study. DNA copy number changes were detected in 12 (75%) of the remaining 16 cases. Twenty-one instances of chromosomal aberrations were detected in total, including : +Iq, +17q, +8q, +20q, -13q, -ll q, -22q, -l p, -1 6q, -8p. The most frequent were +1q, +17q, +8q, -1 3q, similar to other studies. In three cases, we detected -1 3q, which is associated with axillary lymph node metastasis and was reported in one previous study. The mean numbers of chromosomal aberrations per tumor in metastat ic and nonmetastatic tumors was 1.5 and I , respectively. No other association between detected chromosomal aberrations and histopathological and immunohistochemical findings were seen. Conclu sion: Since intermediately to widely invasive carcinomas are more likely to have chromosomal aberrations. CGH can be a valuable prognostic tool. Furt hermo re, CGH can be used to detect targeting molecules within novel amplifications whicb holds the potential for a new therapeutic approac h for intractable cancer.