مطالعه آسيب شناسي بافتي تاثير ويروس آنفلوانزاي تحت تيپ H9N2 در دستگاه تنفسي جوجه هاي SPF

Histopoathological study of the effects of Influenza virus subtype H9N2 on the respiratory system of SPF chickens

Influenza virus causes histopathological injuries in animals and human beings. The main purpose of this study was to determine the effects of int1uenza virus on respiratory tract in chickens infected with avian int1uenza virus, A/chickenlIranl772/2000(H9N2). For this purpose, 20 SPF chickens at the age of 3 weeks were randomly assigned into two equal groups as treatment and control. The treatment group was infected with 107.5ETD50 titer of the virus intranasal and the control group was treated with saline normal in the same manner. After 72 hrs of the experiment, respiratory tissue specimens of the experimental animals were collected and fixed in 10% buffered formalin solution. The prepared microscopic sections with the thickness of 5 micron were stained using H&E method. In comparison to the control group, there were significant mean difference of pathological injury in respiratory tract of the infected group (P<0.005). The results of this study indicate that Influenza virus serotype H9N2 A/chickenlTranl772/2000(H9N2) is able to induce severe pathological injuries in respiratory tract of the infected chickens.