انتقال cDNA گيرنده فعال كننده پراكسيزومي نوع گاما 1 موشي به داخل سلولهاي فيبروبلاست گاوي و بررسي بيان درون سلولي آن به كمك پروتئين گزارشگر فلورسنت سبز

Transfection of mouse PPARgammal cDNA in to the bovine fibroblast cells and analysis of its intracellular expression using EGFP gene reporter

The main aim of this research is to study nuclear localization of mouse PPARyl cDNA into pEGFP-CI. To investigate the nuclear localization of PPARyl protein linked to EGFP marker gene into bovine fibroblast and analysis of intracellular green fluorescency Mouse PPARyl cDNA in a mammalian expression vector (pEGFP-CI) in a chimeric eDNA type, encompassing PPARyl with EGFP eDNA. EGFP marker was used for monitoring. To confirm the intracellular localization of EGFP- PPARyl, bovine fibroblast cells were transfected with the 2.4 flg constructed plasmid and 6 ul Lipofectamine 2000. The related product was entered into the nucleus of bovine fibroblasts after transfection of its cDN A. As expected, chimeric cDNA of EGFP-PPARyl correctly expressed and related protein was dominantly entered to the nuclei of the cells. Thus the recombinant plasmid could be applicable for further studies to unravel the further aspects of PPARy I function. Moreover fusion of EGFP with PPARy I does not hamper the nuclear targeting of PPARy I.