جنين زايي سوماتيكي در سه رقم پنبه Somatic embryogenesis in three cotton (Gossypium hirsutum L.) cultivars (Gossypium hirsutum L

Somatic embryogenesis in three cotton (Gossypium hirsutum L.) cultivars

In cotton (Gossypium hirsutum L.) transformation, for regenerating plants from a single cell, an optimized tissue culture system is necessary. Successful production of transgenic cotton depends on regenerating of many plants; therefore, somatic embryogenesis was investigated for three cotton cultivars. Hypocotyls of cvs. Sahel, Varamin and Coker 312 were isolated and placed on two cotton callus induction media containing MSB medium supplemented with 0.1 mg/l 2,4-D, 0.1 mg/l kinetin, 0.75 g/l MgCl2; and MS medium supplemented with 0.1 mg/l Kinetin and 0.2 mg/l IBA. In medium containing 2,4-D and Kinetin, Sahel and Coker312 explants produced callus earlier than Varamin and the volume of calli were larger, too. The percentage of callus production for Sahel cultivar was higher than Varamin. For embryogenesis induction and maturation of embryos, produced calli were transferred onto MSB medium supplemented with 0.75 g/l MgCl2 and 1.9 g/l KNo3. Numbers of embryogenic calli for Coker312 were higher than Sahel. Germination of somatic embryos for Coker312 and Sahel on embryo germination medium containing MSB supplemented with 0.1 mg/l Zeatin and 2 g/l activated charcoal were earlier than Varamin. Percentage of somatic embryos produced from Coker 312 was higher than Sahel. For rooting, there were no significant differences among cotton cultivars and five rooting media.