مطالعه وسترن بلاتينگ آنتي ژنهاي پروتئيني سوماتيك فوزاريوم سولاني

Western blottinganalysis of protein antigensof Fusarium Solani

Introduction: Western blotting paLlern of somatic protein antigens extracted from fungi can be useful in identification of different species and strains, and also helps to find the infectious agent. This study was undertaken to determine the somatic protein antigens pattern extractedfrom FusariumSolani, MaLerials& Methods: The Fusarium Solani strains UAMH 7419 and UAMH 3317 used in our study to prepare the somatic protein antigens extracts from young mycelia were originally isolated from a patient and the environment in Canada, respectively. Determination of numbers and the protein antigen characteristics was carried out using sodium dodecy sulfate polyacrylamide gel electrophoresis (SDS- PAGE) and western blotting technique. Findb.gs .Analysis of western blotting patterns of somatic proteins from F. Solani lJAMH 7419 and UAMH 3317 demonstrated 16 and 11 antigen bands, respectively. The molecularweights of protein antigens were between 14 to 100 KD. Conclusion :The F. Solani strain UAMH 7419, isolated from the patient, showed more antigenic bands in western blottingthan the F. Solani strain UAMH3317 isolated from the environment.