بررسي فراساختاري اثر فاكتور رشد عصب و فاكتور شبه انسولين بر روي رژنراسيون عصب محيطي موش صحرايي

An ultra structure study on the effects of nerve growth factor and insulin-like growth factor on rat peripheral nerve regeneration

Introduction: Formation of myelin depend s on maturation of the axonal cytoskeleton and mitochondria. Nerve growth factors and insulin-like growth factor can specifically playa role in their regulation. Materials&Methods: This research is an experimental study, Forty eight male rats (200-250 g) were used . After axotomy, 1 ern segment of the scia tic nerve in rats was resected, then the gap was bridged by one of the four methods (polyvinylidene fluoride or PYDF + nerve growth factor + insulin growth factor ·1 , autograft, sham and control ). Sciatic nerve was studied by electron microscope after 4 and 12 weeks of implantation . Findings: After 4 weeks of regeneration , the mean number of unmyelinated axons in autograft and nerve growth factor groups was not significant , but after 12 week s of implantation, the mean number of unmyelinated axons in autograft group was superio r to that of the nerve growth factor group (P