مقايسه روش ايمونوكمي لومينسانس با PCR براي تشخيص ويروس هپاتيت B در بيماران مراجعه كننده به آزمايشگاه بيمارستان بقيه الله

Comparison of Chemiluminescence Immunoassay and PCR for Detection of Hepatitis B Virus among Patients Referred to Baghiatollah Hospital Lab.

Introduction: Hepatitis B virus expresses HBsAg on its envelope.It is a cause of acute infection in infected new-borns and liver disorders such as cancer in human beings . HBsAg is the first serological marker to appear in blood - stream before any clinical signs in patients. It is possible to detect this Ag by immunological methods such as chemiluminescence immunoassay. HBV DNA can be detected by molecular techniques such as PCR in sero-negative patients with known HB disease. Thus, this study is aimed to throw a Comparison between chemiluminescence immunoassay and PCR for detection of Hepatitis B virus. Materials & Methods : Sixty-three serum samples obtained from known HBV patients in Baqyiatallah hospital were used for analysis. Immunological assay and DNA detection were performed by Sandwich chemiluminescence immuno assay and PCR respectively. Findings: The results showed that all the 63 samples (100%) proved positive by PCR , while only 57 cases (90.5%) proved positive and 6 cases (9.5%) negative by Immuno Chemiluminecens Assay. Conclusion: Considering the results obtained from the investigation that shows PCR as more sensitive than Chemiluminescence Immunoassay for diagnosis of HB virus, we should rather suggest using PCR for rapid detection of HB virus in patients than Immuno Chemiluminecens Method.