ارزيابي مقاومت نايسريا مننجايتيديس به عوامل ايمني طبيعي در شرايط آزمايشگاه: مقايسه روش هاي سرمي با روش خون كامل

Ex-vivo assessment ofmeningococcal resistance to natural immunity: serum bactericidal assays versus whole- blood assay

Background and purpose: Literature review indicates that antibiotic resistance of bacteria may be associated with the resistance to host natural immunity. In the previous study we found that all meningococcal isolates of meningitis cases are less sensitive to typical anti-meningococcal antibiotics. In present study, the association of the above characters was analyzed in an ex-vivo "case-control" study. Materials and methods: A randomized controlled trial compared effect two dietary treatments (low glycemic index & low fat) in 46 adults, ages 18-55 years old, BMI >27,who proceed to motahary clinic in shiraz, Iran. Body weight, BMI, WHR (waist/hip ratio), fast and post-prandial Plasma lipid profile (Triacylglycerol, total Cholesterol, LDL-C, HDL-C concentration) level of obese women measured at the beginning and at the end 6 weeks . Results: The resistance of both groups against selected sera were similar in the ratio I :2- by SIC and SBC methods. The growth of "test" and "control" groups in the pooled sera were stopped at the ratios 1:512 and 1:10ʹ24 respectively . In SBA the population of "test" and "control" groups decreased ill parallel to each other. However, the invasive (test) meningococcal strains established slower reduction in WBA. Conclusion: Studies on the correlation between antibiotic resistance of bacteria and the resistance to the natural immunity have ended with different results. According to our experienee, it seems that for ex- vivo investigation ofnatural immunity against meningococci the WBA is more reliable