بررسي ميكروبيولوژيك و حساسيت ضد ميكروبي در موارد كشت مثبت بيماران مبتلا به تب مالت با سرولوژي مثبت

Microbiological study and antimicrobial susceptibilities of brucella isolates in serologic diagnosed cases

Background and purpose: Brucellosis is a zoonotic disease with worldwide distribution that is endemic in Iran . Worldwide, brucellosis remains a major cause of morbidity in humans and domesticated animals. The disease has a wide spectrum of clinical manifestation and can affect a variety of organs and systems. This study focused on blood culture of serologic diagnosed brucellosis and antimicrobial susceptibility test. Materials and methods: In this cross sectional stud y, microbiologic survey was done on a total of 30 serum samples with STA titer of I: 160 or greater and 2ME titer of I :40 or greater, which were presumptive for brucellosis. Blood cultures were done by lysis centrifugation and antimicrobial susceptibility test, against 9 antimicrobial agents by disk method. The data was anal yzed by stata V8.0 software. Results: At the end this study , the blood culture isolation rate was 23.3 %( 7 cases out of 30 patients) and all of the isolates were brucella melitensis. Antimicrobial susceptibility tests showed high in vitro activity of ofloxacin, ciprofloxacin and doxycycline and also, low in vitro activity of streptomycin and cotrirnoxazole. Conclusion: Brucellosis is endemic in Iran . Brucella melitensis was the most common strain of brucella in our patients. Except cotrimoxazole and streptomycin, high in vitro activity was found with other antibrucella agents, especially with ofloxacin, ciprofloxacin and doxycycline