بررسي اثر فاكتورهاي هورمون رشد و مشتق از پلاكت بر روي رشد و تكثير سلولهاي اپي تليال پوست رت

The effects of GH and PDGF on the growth and proliferation of the epithelial cells

Introduction: Epithelial is an important tissue in our body. The human epithelium forms a barrier between the external and internal environment. Natural process of wound healing e.g. ischemia, necrotic tissues and infection is a concept that has been in practice for a long time. Large full thickness wounds donʹt heal spontaneously and are life treating. The closure of large wounds, when there is limited availability of autologous split skin grafts, has been a significant challenge to bums surgeons over the last century. Material & Methods: Rat keratinocytcs were isolated from pups I DIY and cultured according to the to the protocol already described by Duigos et al. Keratinocytes Isolated and were cultured in flask 75cm for 7 days ,then different concentrations of POOF or OH were added daily for 6 days. Finally MTT test were done for all of wells(Case and Control) . Results were analyzed by Excel & SPSS. Results: Results of current study showed that keratinocytes culture were successfully established. There were two kinds of cells after 7 days in vitro. Both of the factors could stimulate cells reproduction. Count of cells between concentrations of 10 - 200 ng of POOF was significant (P