پيوند اتولوگ تخمدان سالم و دست نخورده موش در ماهيچه سريني سطحي

Autologus Transplantation of Intact Mouse Ovaries in Gluteus Supemcialis Muscle

Objective: Anti-cancer therapies frequently lead to ovarian damage and defective fertility . To preserve fertility , cryopreservation and subsequent transplantation of the ovaries has been suggested . The aim of this study was to investigate the survival of follicles in intact intramuscular mouse autologous ovaries , according to the time the ovarian tissue remained In the grafted site. Materials and Methods: Ovaries (n=9) were transplanted intramuscularly into gluteus supe rficialis . These grafted ovaries were removed after one , two and three weeks from the grafted site . A histological examination and counting of follicles was then performed . Some ovaries (n= 3) from the same mice were selected randomly for the control group. Hematoxyline and eosin (H & E) staining was used for follicle counting and TUNEL staining for the examination of apoptosis in grafted tissues. Results: Mean follicular survival was significantly lower in experimental groups compared to the control group (non-grafted) (p