سرواپيدميولوژي ليشمانيوز احشايي به روش آگلوتيناسيون مستقيم DAT در شهرستان بجنورد از استان خراسان شمالي در سال 1386

Seroepidemiological Survey of Visceral Leishmaniasis by Direct Agglutination Test in Bojnoord District, North Khorasan Province in 2007

Background & Objectives: This study oimed to determine of the seroprevolence of viscerol leishmaniosis to design 0 prevention ond control progrom in Bojnoord district. Methods: This wos 0 descriptive and cross sectional study with multistage random sampling method. The study wos carried out on children up to 12 years old and 10% of adultʹs population in 8 rurol oreos of Bojnoord district in 2007. In total, 1608 blood samples were collected to detect onti-Leishmanio antibodies. All the samples were tested by direct agglutination test (DAT). In order to determine Leishmania species, necropsy was performed on four suspected dogs in the studied areas. The species of Leishmania wos determined by RAPD-PCR and PCR-RFLP methods using to amplify the ribosomal internal transcribed spacer 1 (ITS1). Results: Thirty and eight (2.36%) out of 1608 collected blood samples had anti-Leishmania antibodies at titer 1:800 and nine (0.56%) were [ust positive at 1:3200 by DAT. There was no statistically significant difference between female and male seroprevalence (p>Oj05). The seoprevalence in children <=12 years old compared to individuals greater than 12 years old did not show any statistically significant (p>Oj05). Amastigotes were observed in all 4 necropsied dogs. The species of Leishmania isolated from 2 dogs, wos determined os L./nfanfum. Their ITS1 sequences were registered with Accession numbers EU81 0776 and EU81 0777 in NCBI. Conclusions: These findings showed that visceral leishmaniosis has been circulated with low endemicity in Bojnoord district. Therefore an oppropriate monitoring system is needed for health services in this area.