بررسي تغييرات آنزيمي جمعيت هاي آزمايشگاهي .Trichogramma brassicae Bezd در مقايسه با جمعيت هاي طبيعي در مزارع برنج و ذرت مازندران

Comparative Study on Enzymatic Changes in Trichogramma brassicae Bezd., Laboratory and Corn-Rice Field Populationes of Mazandaran Region

In modem bnysystemaiics.buKhemiehal and morphomelric basis are fundamentals olʹ evolutional) taxonomy which its biotechnology shows the inlrapopulaiion evolution in different environmental and mass-rearing situations in comparison with natural host population in agroecosystems. Our research program was based on enzymatic study and in comparison with morphomelric results identification of Trichogramma brassicae Bezd. Parasitoids of rice siemborer (Chi/o supprcssalh WLK.) and corn Mem borer {Osfrinia tuthUaHs \M\pcr\ica HBN.) in different fields of Mazandaran ,also in comparison with different population ol" Trichogramma brassicae feeding on mass-rearing host. Ephcstia kuchniclla Zell. Motphologieal characters of T. brassicae were studied by means of male genitalia and antennae. Electrophoretic technique have been used for enzymatic study. Poly aery lamid gels molded between glass plates.For enzyme polymorphism included 12 populations,20 adults descendants of virgin females in which esterases (EST) were analyzed.The genetic identity distances were calculated based on Nei (1972) method. Based on dendogram, difference between natural and mass-reared populations were observed. Results from enzymatic and moiphomeiiic investigations indicated that there is significant differences (95%-99<7r) between these populations. The efilcaey of biological control is dependent on the quality of the T. hraxxicae .Quality control has always been an important factor in bioeontrol.Thc selection of suitable and adaptive species.quality of mass-reared and climate conditions at the time of release are the main factors that determine the efficacy of using Trichogramma brassicae in Mazandaran. In the other hand for utmost effect, we have to improve the quality control