تغييرات ميزان رسوراترول در برگ و ميوه و اثر احتمالي متيل جازمونات بر آن در دو رقم انگور (.Vitis vinifera L) ايراني

Effect of Methyl Jasmonate on Resveratrol Production in Leaf and Fruit of Two Iranian Grape (Vitis vinifera L.) Cultivars

Resveratrol (5,3,4ʹ- trihyroxystilbene) is a valuable polyphenolic compound which prevents cancer, heart and vascular diseases in human. In this research, the compound was extracted from leaf and fruit tissues of two Iranian grape cultivars (Rajabie Sefide Shiraz and Keshmeshie Ghermez) using a general extraction method with some modification, and analyzed quantitatively by High Performance Liquid Chromatography (HPLC). Resveratrol content in leaf and fruit of Rajabie Sefide Shiraz cultivar was higher than Keshmeshie Ghermez and its amount in the fruits of both cultivars was observed to be greater than their leaves. The accumulation of Resveratrol at the early stage of fruit growth (4 weeks after full bloom) was higher than the subsequent stages of fruit growth and development (8 weeks after full bloom and ripened fruit) in both cultivars. This compound gradually decreased from the beginning of fruit set to the complete stage of growth and development and reached the lowest level when the fruits were ripe. Leaves and fruits of both cultivars were treated with methyl jasmonate (1 mM), then the rate of resveratrol was measured after treatments. It was observed that the amount of this compound was significantly increased in treated samples when compared with the control; however, the difference diminished later. The fruits of both cultivars responded better to the treatments 4 weeks after full bloom.