تنوع و روابط ژنتيكي برخي ژنوتيپ هاي انگور (.Vitis vinifera L) استان اصفهان با استفاده از نشانگرهاي RAPD

Analysis of Genetic Relatedness and Variation Among Some Genotypes of Grapevine Grown in Isfahan Province Using Randomly Amplified Polymorphic DNA Markers

Grapevine (Vitis vinifera L.) is a clonally propagated major fruit crop. In grapevine, identification of genotypes with amplographical features is often based on mature plant characteristics that may be affected by environmental conditions. This approach lacks objectivity and reliability. Recently, molecular markers have proved to be supplementary techniques to analyze genetic diversity and examine genetic relationships existing between cultivars in a range of horticultural crops. In this study, twenty genotypes from grapevine (V.vinifera species) grown in Isfahan province were characterized by RAPD technique to understand the extent of diversity and relatedness. Fifty random primers were used for the RAPD study. Of those, twenty four informative primers which generated reproducible polymorphic bands were used for grouping the genotypes. PCR products of the genotypesʹgenome revealed a total of 315 bands, out of which 282 were found to be polymorphic. Average number of 13 bands was obtained per primer and the amplification produced ranged in size from 300 bp to 3000 bp. The dendrogram constructed using UPGMA cluster analysis differentiated the genotypes into two major clusters, nineteen in one group and Madar-o-Bache genotype has been placed in a separate one, indicating its high genetic diversity compared to the rest of the genotypes. Intra- clustering within cluster A grouped the genotypes in four sub-clusters as expected from their genetic background. The results of the study revealed that the RAPD technique is a relevant technique to determine genetic diversity, genomic analysis and to examine genetic relationship in grapevines.