بررسي تغييرات ميزان پروتئين ARG طي پيشرفت بدخيمي لنفوماي منتشر سلول بزرگ B

The evaluation of ARG expression in DLBCL stag¬ing

ARG is a proto-oncogene and a member of a tyrosine kinase proteins family. It has great importance in many cancers, but there is no con­clusive evidence on its role in DLBCL staging. The aim of this study was to evaluate importance of ARG, in this cancerʹs staging. Sixteen DLBCL and 4 reactive lymph node (as control group) sam­ples, after staging using Ann Arbor staging system, were used in this study. Formaldehyde fixed paraffin embedded blocks were prepared from the samples. After the sectioning, all samples were hybridized with primary antibodies against ARG and GAPDH (as control house keep keeping gene). The slides were then washed and hybridized with fluorescently labeled secondary antibodies (ARG, green labeled and GAPDH, red). After capturing the pictures using CCD camera, the in­tensity of green and red colures was measured and the ratio between green/red, that demonstrate changes in ARG expression, were calcu­lated. The mean ratio of green/red (ARG expression) was significantly dif­ferent between reactive lymph node, stage I, II and III of DLBCL. ARG expression was different between all groups except for between stage III and VI of DLBCL. The observed changes in ARG expression is a potential biomarker for DLBCL staging. In addition specific inhibitors of ARG can be consid­ered as new chemotherapy agents in the DLBCL treatment