توليد گره تثبيت نيتروژن در ريشه هاي گندم تحت تاثير عوامل مختلف توليد كننده گره و تلقيح با باكتري

The Study of Nitrogen-Fixing Activity on Wheat Roots with Different Treatment Condition (Inoculation with Nodulation Agents and Diazotrophic Bacteria)

In this study, isolated strains of nitrogen fixing bacteria and pectynolytic bacteria which can able to create paranodule on wheat seedling were selected from soils. Then the best nitrogen fixing bacteria and high capable peptonize enzyme production bacteria were chosen. 2.4-D was used as Abiological agent (Aa) and the best pectolitic bacteria as Biological agent (Ba) (Bacillus polymyxa Strain 43) for study formation Para - nodule on wheat seeding and also used diazotroph bacteria for inoculation Wheat Seeding. Nitrogen-fixation Activity on wheat roots was measured in dynamics on the 8th, 10th, 14th and 22nd days of plant growth. The experiment was carried out in 9 variants as following. 1) Wheat without any treatment (control) 2) Wheat + Aa 3) Wheat + Ba 4) Wheat inoculated + mixed cultures of diazotrophic bacteria (Arthrobacter sp + Xanthomonas sp). 5) Wheat + Bacillus polymyxa Strain 42 6) Treatment 4 +Aa 7) Treatment 5 + Aa 8) Treatment 4 + Ba 9) Treatment 5 + Ba. The formation of P- nodules on wheat roots was observed in the 4-5th day of plant growth. Their diameters were about 0.5-1 mm. It was shown that the significant differences in the number of P-nodules and also the rate of nitrogen fixing activity on wheat roots depending on the treatments. The maximum nitrogen-fixing level was recorded after wheat plant inoculation with mixed culture (Arthrobacter sp + Xanthomona sp) on the 14th day of plant growth.