بررسي اثرات آگونيست GnRH بر روي لوله هاي اسپرم ساز موش هاي صحرايي نابالغ

EFFECTS OF GNRH-A ADMINISTRATION ON SEMINIFEROUS TUBULES OF IMMATURE RATS

Background & Aims: Spermatogenesis dependent to Hypophyseal Gonadotropin hormones. Secretion of Gonadotropin hormones is controlled by Hypothalamic Gonadotropin releasing hormones. GnRH analogues disregulated Hypophyseal-Gonadal axis. The aim of the present study was to evaluate the effects of GnRH-agonist administration on seminiferous tubules in immature rats. Materials & Methods: In this study the immature rats (aged 25 days) were divided into two experimental groups (n=5) and two control groups (n=5). Subcutaneous injection of GnRH-a (0.lmg/ml) and NaCl (physiologic Serum) were carried out every 24 hours for 5 days, respectively in experimental and control groups. After dissection, the testis were excised and processed for histological study 5Mm serial sections. Results: The results showed that there was not significant difference in diameter of tubules between first experimental group and control groups, but there was significant difference in lumen diameter, thickness of epithelium and the number of cell layers in seminiferrous tubules. Furthermore, there was not significant difference in diameter of tubules between second experimental group and two control groups, but it is significant in lumen diameter, thickness of epithelium and the number of cell layers (p<0.05) In addition the morphometric findings showed that there was not significant differences in diameter of seminiferrous tubules between two control groups, but the difference in lumen diameter, thickness of epithelium and number of cell layers was significant. Conclusion: We found that GnRH-agonist (buserelin) induces spermatogenesis in immature male rats.