شماره ركورد :
421095
عنوان مقاله :
اثر مهاري گابا بر ترشح انسولين تحريك شده توسط گلوكز از جزاير لانگرهانس جدا شده ي پانكراس موش صحرايي
عنوان به زبان ديگر :
Inhibitory Effect of GABA on Glucose- Stimulated Insulin Secretion from Isolated Islets of Langerhans in Rat
پديد آورندگان :
فرجي شهريور، فرزانه نويسنده دانشگاه علوم پزشكي شهيد بهشتي تهران , , قاسمي ، اصغر نويسنده دانشگاه علوم پزشكي شهيد بهشتي تهران Ghasemi, A , خسروي، مريم نويسنده دانشگاه علوم پزشكي شهيد بهشتي Khosravi , M , معتمدي، فرشته نويسنده دانشكده علوم- دانشگاه آزاد اسلامي واحد تهران شمال Motamedi , F , زاهدي اصل، صالح نويسنده دانشگاه علوم پزشكي شهيد بهشتي تهران Zahesi Asl, S
اطلاعات موجودي :
دو ماهنامه سال 1387 شماره 37
رتبه نشريه :
علمي پژوهشي
تعداد صفحه :
8
از صفحه :
59
تا صفحه :
66
كليدواژه :
باكلوفن , ساكلوفن , گابا , انسولين , گلوكز , جزاير لانگرهانس , موش صحرايي
چكيده لاتين :
Introduction: Gamma-amino butyric acid (GABA), an amino acid, present in some inhibitory neurons of the central nervous system, is also found in relatively high levels in the islets of Langerhans. Results of different studies concerning the effect of GABA on insulin secretion are controversial. The aim of this study was to determine the role of GABA and GABAB receptors on glucose-stimulated insulin secretion in isolated islets of rats. Materials and Methods: The collagenase digestion technique was used to isolate the islets from pancreata of 45 male Wistar rats (200-250g). Insulin secretion was assessed in eight islets in each cup exposed to different concentrations of glucose (8.3 and 16.7 mM) in the presence or absence of GABA(25, 50, 100 pM), baclofen (10, 20, 50 jjM) (GABAB agonist) and saclofen (50,100 pM) (GABAB antagonist). Insulin concentration was measured by the ELISA method. Insulin release was reported as mean±SEM |iU/islet/50min and p values of <0.05 were considered significant. Results: GABA inhibited glucose (8.3 and 16.7 mM)-induced insulin secretion in isolated islets (P<0.05). Different concentrations of baclofen had no significant effect on glucose-induced insulin secretion; however glucose (16.7mM) stimulated insulin secretion in the presence of 100 jjM saclofen (91 ±8.8 pU/islet/60min) was significantly (p<0.05) higher compared to insulin secretion stimulated by 16.7mM glucose alone (67.7±2.58 pU/islet/60min). Conclusion: These findings indicate that GABA has an inhibitory effect on glucose-induced insulin secretion; and therefore it may play a regulatory role in insulin secretion. This effect needs to be taken in to account in the pathophysiology of diabetes.
سال انتشار :
1387
عنوان نشريه :
غدد درون ريز و متابوليسم ايران
عنوان نشريه :
غدد درون ريز و متابوليسم ايران
اطلاعات موجودي :
دوماهنامه با شماره پیاپی 37 سال 1387
كلمات كليدي :
#تست#آزمون###امتحان
لينک به اين مدرک :
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