اثر آلومينيوم بر رشد سلولهاي آستروسيت جنين انسان در محيط كشت و بيان ژنهاي درگير در آپوپتوژ

Effects of Aluminum on Cultured Human Embryonic Astrocytes and Apoptotic Gene Expression

Introduction: Aluminum is the third most abundant element in the earthʹs crust .It plays a role in several neurodegenerative conditions including Alzheimerʹs disease. In this study, we investigated the effects of aluminum on human embryonic astrocytes. Materials & methods: In this experimental study, we applied MTT techniques to investigate the effects of 3,9, and 12 days exposure to aluminum on astrocyte viability . Then, we used immunocytochemical techniques to identify apoptotic gene expression changes induced by aluminum. We used Stata software to analyze the data. Findings: Our results showed that 3-days exposure to 1 and 1.5 mM caused a reduction in astrocytes viability, (P<0.01, P<0.03). Low levels of aluminum (500 and 100 µM) needed long-term exposure to become toxic to astrocytes, (9 days for 500 and 12 days for 100 µM) (P=0.000). Aluminum didnʹt show any effects on apoptotic gene expression. Conclusion: Long-term exposure, even to low levels of aluminum, was toxic for human embryonic astrocytes, but it seems that aluminum does not alter apoptotic gene expression