تأثير داروهاي ضد قارچي فلوكونازول، ايتراكونازول و كتوكونازول به صورت منفرد و مخلوط با يكديگر بر روي مخمرهاي بيماريزا

Antifungal effects of fluconazole, itraconazole and ketoconazole in intact forms and also combinations to each other against some pathogenic yeasts

Background and Aim: In recent years, fungal systematic infections due to pathogenic yeast have been considered as the most important causes of dead. Current antifungal therapies using routine antifungal drugs have not completely effective. In last decade, different formulations of combined antifungal have been tested against some pathogenic fungi which shown good synergistic effects. In present work, antifungal effects of some azoles e.g. fluconazole (flu) Itraconazole (it) and ketoconazole (kcz) were studied in intact forms and also in combinations to each other against some pathogenic yeast including Candida albicans PTCC5057, Candida dubliniensis CD36, Cryptococcus neoformance CNE1 and Malassezia furfur MF1, in vitro. Materials and Methods: In present work, antifungal effects of some azoles e.g. fluconazole (flu) Itraconazole (it) and ketoconazole (kcz) were studied in intact forms and also in combinations to each other against some pathogenic yeast including Candida albicans PTCC5057, Candida dubliniensis CD36, Cryptococcus neoformance CNE1 and Malassezia furfur MF1, in vitro. The microdilution method was used for assessing antifungal susceptibility of above-mentioned compounds in two culture media sabouraud dextrose broth (for all fungi except M. furfur) and modified Dixon broth (for only M. furfur). MIC and MFC values were calculated for each compound based on comparing colony counts of test and control samples. Results: On the basis of obtained results, all drugs were inhibited the growth of all fungi tested in a dose-dependent manner. MIC ranges of Flu, It and Kcz for C. albicans (1-256, 0.5-16, 0.5-64 microgram/ml), Candida dubliniensis (0.5-32, 0.125-8, 0.5-8 microgram/ml), C. neoformance (0.5-64, 0.125-32, 0.25-64 microgram/mL) and M. furfur (1-128, 1-64, 0.5-32 microgram/mL) were obtained, respectively. Results: Our results showed that the most effective drugs against Candida dubliniensis were Itraconazole and Fluconazole in intact forms and in combination to each other. Fluconazole and Ketoconazole in combination to each other are the most effective drugs against Malassezia furfur and Cryptococcus neoformans. Also Ketoconazole and Itraconazole in combination to each other are the most effective drugs against Candida dubliniensis, Cryptococcus neoformans and Malassezia furfur (P<0.05).