بررسي ميزان فراواني و شناسايي عوامل قارچي تخم و لارو تاسماهي ايراني مركز تكثير و پرورش ماهيان خاوياري شهيد بهشتي سال 1386

Identification and abundance of fungal flora in egg, and larvae of Acipenser persicus in Shahid Beheshti Sturgeon Rearing Center (2007)

studied. Totally, 270 specimens from Shahid Beheshti Sturgeon Rearing Center were examined. A heterogeneous solution from samples was prepared and inoculated on culture media SDA+C and CMA+C in lines under sterile conditions. Wet mounts were prepared for the identification of Saprolegnia sp. and the inoculants were cultured on culture media GP containing gentamycin and chloramphenicol. We found Penicillium, Cladosporium, Fusarium, Yeast, Mucor sp., Aspergillus niger and Paecilomuces on egg samples in order of frequency and in water samples we observed Penicillium, Cladosporium, Fusarium, Yeast, Mucor spAspergillus niger, and Paecilomyces. Fungal species identified in yolk sac larvae included Penicilliium sp., Cladosporium sp., Fusarium sp. ^Alternaria sp., Yeast and in water samples we found Penicillium, Cladosporium, Fusarium, and Yeast, while in larvae we saw Cladosporium sp., Penicilliium sp., Fusarium sp., Alternaria sp., Aspergilus fumigatus, Yeast and Mucor spp. In water samples containing larvae we were able to identify Cladosporium sp., Penicilliium sp., Fusarium sp., Yeast and Aspergilus niger. Fungal species such as Cladosporium sp., Penicillium, Fusarium, Yeast and Saprolegnia were detected in all four sampling mediums. T-test indicated no significant differences in total counts (colonies/2 plates in all samples) in eggs (15.08±3.51 colony forming unit; CFU) and in water (15.91± 2.63) samples. However, t-test indicated significant differences in total counts in yolk sac larvae (5.33±1.05) and in water (11.77±2.39) samples. T-test showed no significant differences in total counts of larvae (32 ±12.46) and water (31.11±12.79) samples.