بررسي اثر اتانول بر ساختمان ميكروسكوپي ريه موش بالغ

The study of ethanol effects on microscopic structure of the lung in adult mouse

Background and Aim: Ethanol is a well-known substance with extensive medical and industrial applications. It is used also as a drinking and has dangerous side effects on several organs of the body. Effects of ethanol on some organs of the body have been studied but few experimental studies have been carried out on lung. The purpose of this research was to study the effects of ethanol on histological changes of lung structure after ethanol consumption in adult mouse. Materials and methods: Twenty adult Male and female, 3 month age mice, were selected and divided randomly into two experimental and control groups. Diluted ethanol was injected intraperitoneally, once daily, for 21 days in the experimental group. Control group received normal saline simultaneously. After 21 days ethanol consumption, mice were anesthetized and lungs were removed. Specimen were immediately washed in normal saline and fixed in formalin, dehydrated with an ascending ethanol series, cleared with xylene and then embedded in paraffin. The paraffin blocks were cut into sections of 5jnm thickness. Sections were stained with H&E and were studied by light microscope. Results: Congestion in blood vessels of the septal alveolar, accompanied by bleeding, moderate and severe inflammatory cells infiltration, mainly polymorphonuclear neutrophil^ were seen on septal alveolar tissues by light microscopy. Focal intraalveolar bleeding was seen in some areas of the lung as well. Conclusion: This study showed that ethanol may have destructive effects on lung tissues such as blood congestion, bleeding and inflammation.