بررسي پارامترهاي اسپرم در موش NMRI نر تيمار شده با روغن ذرت

Survey Sperm Parameter of Mouse Treated with Corn Oil

Purpose: The purpose of this study was to evaluated the effect of corn oil on mouse sperm count , motility .viability,quality of chromosome,testicular spermatid number per gram testis (TSN).anddaily sperm production (DSP) Materials a nd Methods: The protocol for corn oil adminstration was that male NMRI mice(the age groupe of 4weeks) received 100 and 150 pi corn oil orally for 14 days. The control group did not receive corn oil. All animals were weighed on the first and terminal day of experiment and then killed by cervical dislocation. The right and left testis weight without fat were recorded for each animal. sperm analysis was evaluated the end of this time. Epididymal spermatozoa from adult mice were collected in T6 medium supplemented with 4mg/kg BSA and the preserved sample at incubator 37°c for 1 hour .All the samples were assessed according to world health organization criteria. Abnormal morphology was assessed using papanicula staining. Quality of choromosome sperm also was assessed using anilin-blue staining. After detached capsule right testis,the paranchyma was homogenized at low speed for 4 minutes in 2ml of 0.4/. Nacl solution. Homogenization-resistant spermatid heads were counted by aimproved neubar haemocytometer. Number of spermatid were expressed at TSN. DSP was calculated by dividing the total number of spermatid per gram testis by 4/84(The duration of step 14-16 spermatids in the mouse seminiferous epithelial cycle.)One-way ANOVA and Kruscal-Walis H were utilized on parameters. Results: The results showed that 100 and 150 pi corn oil daily adminstration decrease count,viability, motility, abnormal morphology and quality of chromosome epididymal sperm ,TSN.DSP,and body weight mouse compared the control group,but there was not seen significant difference(p>0.05).It is no effect on testis weight. Conclusion: The result of this study demonstrated that corn oil admistration is low negative effect on parameters sperm mouse. It is good solvent for reproductive system research studies.