بررسي تاثير اسانس گياه اسطوخودوس بر تريكوموناس واژيناليس در محيط In Vitro

Investigation of Anti Trichomonas Vaginalis Activity of Lavandula angyustifolia Essential Oil in Invitro Media

Introduction: Trichomoniasis is considered a common inter-sexual disease caused by trichomonas vaginalis parasite all over the world. Thus, considering the increasing reports of its spread as well as its resistance to metronidazole, it seemed necessary to find a possible herbal drug for the case. Accordingly, An In vitro investigation was carried out to determine the anti trichomonas vaginalis activity of essential oil of Lavandula angyustifolia which is commonly used in traditional medicine. Materials & methods: Parasite collection was accomplished by obtaining contaminated soap of vagina from trichomonal women as well as cultivating the Trichomonas Modified CPLM Medium Base(TMCPLM). Identification was done through direct smear. Essential oil was prepared by hydro-distillation; then, the parasites were added to the 10 test-tubes containing TMCPLM, metronidazole, dimetyl solfoxide(DMSO), and essential oil with concentration of (0.1,0.01,0.001) to determine the effects of these concentrations in 15 min. interval times for 6 hours. Findings: Findings suggested that Trichomonas vaginalis could remain alive in Trichomona Modified CPLM Medium Base for 48 hours, in presence of metronidazole for 2 hours and TMCPLM for 5 hours. Also, the results indicated that essential oils at concentration of 0.1 and 0.01 were effective at the beginning of inoculation as well as at the concentration of 0.001 hours respectively. Conclusion: The investing authors suggest, further investigations to be carried out to check a more exact effect of anti- trichomonas vaginalis activity of Lavandula angyustifolia essential oil under In vivo condition to recommend it as a therapeutic medicine, if positive results proved.