بررسي تنوع ژنتيكي لاين هاي ژاپني كرم ابريشم ايران با استفاده از نشانگرهاي تفاوت طول قطعات تكثير شده (AFLP)

Study of genetic diversity in japanese lines of Iranian silkworm population using AFLP markers

Genetic diversity of three lines of Iranian Silkworm (Japanies Lines) was assessed, using the AFLP markers. Samples (30 Larvae at 3 rd day of 5th instars from each line) were collected from Silkworm Research Institute (Rasht- Pasikhan). Genomic DNA was extracted from sericigence glands using phenol- chloroform method. In this Study ten Pstl - TaqI primers combinations were used from which that 199 AFLP Polymorphic markers were generated. The dendrogram was constructed using the UPGMA method showed two groups, where Lines 31 and 103 were located in one group and line 107 in the other group. Average genetic diversity for total population and Primers was 0.3692. The lowest genetic diversity (0.1088) was observed for Line 31 and minimum was (0.1484) for Line 103. The maximum and minimum unbiased genetic distance was observed between Lines 31 and 107, and between Lines 31 and 103, respectively. Maximum and Minimum Polymorphism was observed in Lines 103 and 31, respectively.