بررسي اثر ضد ميكروبي و تعيين ميزان تركيبات فنولي، فلاونوئيدي و فلاونولي عصاره اتانولي گل ميموني (Scrophularia striata)

Antimicrobial effects and evaluation of total phenols, flavonoids and flavonols contents of ethanolic extracts of Scrophularia striata

Background and aim: Phenolic and flavonoid contents of plants have usually antibacterial activities. The aim of this study was to evaluate antimicrobial effects and total phenols, flavonoids and flavonols contents of ethanolic extracts of Scrophularia striata. Methods: In this experimental study, ethanolic extract of Scrophularia striata was prepared and examined for antibacterial activities on Ecoli O157: H7, using the macrodilution method. Amikacin (30 µg) was used as an antimicrobial reference. In addition, total phenolic components were determined by Folin-Ciocaltu method and total flavonoid and flavonol components were determined by aluminium chloride method. Results: The phenol content in the ethanolic extracts of Scrophularia striata was 180±6.66 mg/g. Flavonoid and flavonol contents in the extract were 100±6.66 and 74±3.6 mg/g, respectively. The Minimum Inhibitory Concentration (MIC) was 90 mg/ml while the Minimum Bacteriocidal Concentration (MBC) was 100 mg/ml. Conclusion: The findings of this study show that ethanolic extracts of Scrophularia striata have antibacterial effects on Ecoli O157: H7. More works, such as clinical examinations and other extract methods are needed to unravel for the antimicrobial effects of this plant.