بررسي اثر داروي ضد قارچي (گريزئوفولوين) بر بيان ژن كيتين سنتاز در قارچ ترايكوفايتون روبروم

Study of antifungal drug (Griseofulvin) effect on the expression of chitin synthase in Trichophyton rubrum

Trichophyton rubrum is one of the anthropophagic dermatophytes with worldwide distribution. This fungus is a common causative agent of tinea cruris, tinea corporis, tinea manuum and tinea pedis. Current laboratory methods are main methods to study this fungus, even though taking a long time. In spite of many studies in the field of cellular and molecular biology, molecular investigations on dermatophyte fungi are very few and limited. Nowadays resistance had been seen against antifungal drugs. Producing of new drugs or accession to suitable efficacy of existing drugs require more study and finding in the field of molecular biology. In this fundamental study, serial dilutions of griseofulvin with fungal growth were prepared and compared with control sample that was not affected by any drugs. RNAs were then extracted. Afterwards Multiplex-PCR was performed simultaneously for chitin synthase and |Xg/ml beta actin genes in each one of three samples. Microscopically, rate of shortened and twisted mycelia in 0.02 dilution of griseofulvin was much higher than in 0.006 fig/ml dilutions. But regarding the gene expression of chitin synthesis, there was no difference between the control sample and ones affected by griseofulvin.