بررسي چند شكلي ژن لپتين در گاوهاي نر هلشتاين ايران

Associations Between Leptin Gene and Milk Production in Holstein Cattle of Iran

To study the polymorphism of bovine lepton gene, 59 Iranian proven Holstein bulls are genotyped. The PCR was used to amplify a 581 bp fragment including part of intone 1, axon 2 and part of intone 2. Digestion of PCR products were performed with Bsec l( T^CGAT ). The studied polymorphism is an A to T substitution at position 413 of amplified fragment (in axon 2) resulting in Tyrosine to phenylalanine substitution. In wild type (A allele ), the amplified fragment is digested and give two fragments of 412bp and 169bp. In mutant type (T allele), the amplified fragment is not digested. In present study, the allele frequency was estimated 99.15 and 0.85 percentage for A and T allele, respectively. The results of other studies have also shown low frequency of mutant allele (T) in different breeds.