بررسي فعاليت ضد باكتري عصاره گياه شون (Sambucus ebulus) عليه دو باكتري Staphylococcus aureus ATCC 1341 و Pseudomonas aeruginosa ATCC 2785

Survey of antibacterial activity of Sambucus ebulus extracts against Staphylococcus aureus ATCC 1341 and Pseudomonas aeruginosa ATCC 2785

Nosocomial infections causes by multi resistant bacteria are a widespread problemʹespecially in intensive care units (ICU) in hospitals. Irregular using of antibiotics exhibits resistance in bacterial species and there is a need to search for alternatives productsʹ including natural plant extracts to combat infection disease. Guilan province is one of the most important and valuable places in number and variety of plants for this purpose. In this study antibacterial activity of Sambucus ebulus extracts against Staphylococcus aureus ATCC 1341and Pseudomonas aeruginosa ATCC 2785 were studied. Different extracts were obtained form diverse part of plant using solvents such as methanol, ethanol and chloroform. Antibacterial activities were tested invitro against bacteria by Agar Disk Diffusion method. The results indicated that three extracts of leaf ʹ flower and fruits of S. ebulus showed inhibition zones against S.aureus ATCC 1341 about 10-12mmʹ 11-14mm and 11-13mm, respectively. By using K2 examination no significant difference were seen from those of controls (p<0.05). Also, no inhibition zones were observed against P.aeruginosa ATCC 2785. These results showed that the compounds in this plant have antibacterial activity against S.aureus ATCC 1341. This may be due to general physiological different in the membrane constitution of gram negative and gram-positive bacteria. P.aeruginosa ATCC 2785 cell membrane is impermeable to most antibacterial compounds and Sambucus ebulus extracts are not useful against this bacterium.