پديد آورندگان :
شيرازي، محمدحسن نويسنده دانشگاه علوم پزشكي تهران,دانشكده بهداشت و انستيتو تحقيقات بهداشتي; Shirazi, MM , سيروس، مهراندخت نويسنده دانشگاه علوم پزشكي تهران,دانشكده بهداشت و انستيتو تحقيقات بهداشتي; Sirous, M , اشراقي، سعيد نويسنده دانشگاه علوم پزشكي تهران,دانشكده بهداشت و انستيتو تحقيقات بهداشتي; Eshraghi, S.S. , فلاح مهرآبادي، جليل نويسنده انستيتو بيوانفورماتيك عصر نوين,; Fallah-Mehrabadi, J , ابراهيمي درياني، ناصر نويسنده دانشگاه علوم پزشكي تهران,دانشكده پزشكي; Ebrahimi-Daryani, N , پورمند، محمدرضا نويسنده دانشگاه علوم پزشكي تهران,دانشكده بهداشت و انستيتو تحقيقات بهداشتي; Pourmand, P , سلطان دلال، محمدمهدي نويسنده دانشگاه علوم پزشكي تهران,دانشكده بهداشت و انستيتو تحقيقات بهداشتي; Soltan-dallal, M.M , حاجي خاني، سارا نويسنده دانشگاه علوم پزشكي تهران,دانشكده بهداشت و انستيتو تحقيقات بهداشتي; Hajikhani, S
كليدواژه :
مترونيدازول , ToIC , هليكوباكترپيلوري , پمپ افلاكس
چكيده لاتين :
Background: The presence of antibiotic resistance has been reported in H.pylori and it is a major cause of treatment failure. Five families of multidrug efflux pumps are defined in bacteria and resistance-nodulation-division (RND) pumps are found mainly in gram negative bacteria. TolC is one of RND pump components and play a critical role in drug resistance. It hasnʹt been established that RND family has a role in drug resistance in H.pylori. In this study, we assessed the role of two efflux genes in resistant to metronidazole in H.pylori by evaluation of overexpression TolC genes by RT-PCR method. Methods: In five metronidazole resistant strains of H.pylori, total RNA was extracted. RNA treated with DNase and RNA reverse transcribed to cDNA. Aliquots of the cDNA solution were assayed by RT- PCR for HP0605 and HP0971 genes. The levels of mRNA expression were evaluated by densitometry analysis. Results: All five strains displayed overexpression for HP0605 basis of increased concentration of metronidazole. Three strains showed transcripts for HP0971. One of these had transcripts for HP0971 only in Metronidazole concentration equaled to 16Mg/ml but two strains overexpressed adapt to increase concentration of metronidazole. Conclusion: According to current study, HP0605 and HP0971 genes overexpressed due to increase metronidazole. So, increasing of Metronidazole affects in H.pyloriʹs efflux system in transcription level.
