جداسازي، كلونينگ و تعيين توالي ژن تنظيمي claR از استرپتومايسس كلاولي جروس سويه ايراني 1705 PTCC

Isolation, cloning and sequencing of claR regulatory gene of Streptomyces clavuligerus in Iranian Strain PTCC1705

S. clavuligerus is a well known actinomycet for producing a variety of Clavulanic acid beta-Iactam antibiotics, especially clavulanic acid. Clavulanic acid, a potent ~-lactamase inhibitor, is used synergically in combination with penicillin and cephalosporin to inhibit this ~- lactam antibioticʹs inactivation. The claR gene, in the clavulanic acid gene cluster, encoding a transcriptional activator, regulates the late steps in the clavulanic acid pathway. All patents about clavulanic cid production are based on performed researches on claR gene of S. clavuligerus DSM738. Here we have determined claR gene sequence of S. clavuligerus PTCC1705 following its cloning on pMA: :hyg vector.The obtained results show the similarity between claR gene sequence of both strains. In future, subsequent S. clavuligerus transformation with these newly created constructs will make different transgenic or genetically manipulated streptomyces to study the effect of claR amount on clavulanic acid production. Also, genetic .manipulation of claR using different methods, such as site directed mutagenesis. will be done in future using these isolated and confirmed genes.