بررسي اثر اسيد سيتريك و پروبيوتيك بر ميكروفلور دستگاه گوارش زنبور عسل

Effect of citric acid and probiotic on the microflora of honey bee digestive tract

Antibiotics are used for treatment of honey bee diseases. Antibiotics lower the numbers of beneficial bacteria in the body. Antibiotics disrupt normal microflora balance of digestive tract. Antibiotic use in food animal production has been associated with the emergence of antibiotic-resistant strains of bacteria. In response to these practices and attendant problems, several organizations have called for restrictions on antibiotic use in food animal production. For this reason, nutritional additives were used to reduce of antibiotics. Two of these additives are probiotics and citric acid. Both of them are used in honey bee nutrition and have antibacterial activities. Probiotics are defined as live microorganisms, including Lactobacillus species, Bifidobacterium species and yeasts that may beneficially affect the host upon ingestion by improving the balance of the intestinal microflora. The objective of the present study was to determine the effect of Saccharomyces cerevis iae as a probiotic organism and citric acid as an intestine acidifying factor that restrict the growth of intestinal bacteria on the microflora of honey bee digestive tract. To achieve this, bacteria of honey bee digestive tract were isolated and identified. Then, the effect of citric acid and probiotics were investigated. The results showed that citric acid is effective on bacteria of honey bee digestive tract and its MIC and probiotic Saccharomyces cerevisiae reduce the number of bacteria of honey bee digestive tract.